In vitro culture studies on Hemigraphis alternata (Burm.f.) T. Ander. (Acanthaceae): A Potential medicinal herb
- Country : India
- Subject : Botany
Abstract
The current situation of medicinal plants and increasing demand of plant derived drugs suggest an immediate need to conserve our medicinal plant resources. The present study attempted to develop in vitro micropropagation protocol for the important medicinal plant Hemigraphis alternata (Burm.f.) T. Ander. From this experiment it is clear that higher amount of cytokinins and lower auxin induce shoot regeneration and high amount of auxin and lower amount of cytokinins induce root regeneration. Similarly the most effective shoot regeneration was found in the M.S. medium supplemented with KIN 2 mg/l. It shows an effective rooting in the ¼ strength M.S. medium supplemented with NAA 0.5 mg/l. large numbers of multiple shoots were also formed in the M.S. medium is supplemented with KIN 2 mg/l.
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