Effect of Extraction Solvents on the Phytoconstituents of Different Parts of Wedelia Trilobata L. - A Tropical Perennial Medicinal Herb

Abin Antony, Satheesh George

Effect of Extraction Solvents on the Phytoconstituents of Different Parts of Wedelia Trilobata L. - A Tropical Perennial Medicinal Herb



Abstract

The objective of the present study was to evaluate the effect of extraction solvents such as n-Hexane, Petroleum ether, Chloroform, Methanol and aqueous Methanol (Methanol: Water, 50:50 v/v) on phytochemicals of different parts like leaf, stem and flower of Wedelia trilobata using chromatographic studies. The chemical pattern of different extracts was compared using Thin Layer Chromatography (TLC). Variations were observed in terms of number of bands and band intensity which indicate the qualitative and quantitative divergence in chemical constituents. Chloroform extracts of leaf, stem and flower showed highest number of phytochemicals than Hydro-alcohol, Methanol, Petroleum ether and n-Hexane extracts. Due to the higher number of phytochemicals of extract, use of Chloroform for the preparation of herbal formulations can be considered after proper phytochemical characterization, identification of bioactive compounds and quality standard tests and it may lead to the judicious use of raw materials.

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