Antifungal Activity of the Methanol Tuber Extract of Dioscorea Dumetorum (Pax)
- Author Ugochukwu M. Okezie
- Co-Author Chika J. Ifediba, Felix A. Onyegbule, T.H. Gugu, T.C Egbuim, M.C Ugwu
- DOI
- Country : Nigeria
- Subject : Pharmacognosy
Keywords : Dermatophyte, Candida albicans, Aspergillus niger, Dioscorea dumetorum (Pax),
Phytochemical screening, Secondary metabolites
Abstract
The antifungal activity of the methanol tuber extract of Dioscorea dumetorum (Pax) was investigated using standard techniques. The phytochemical screening revealed the presence of biologically active secondary metabolites (alkaloid, tannin, saponin and flavonoid). The methanol crude extract contained 19.4% of tannin, 2.8% of alkaloid, and 0.9% Saponin. These secondary metabolites have been reported by various authors to possess significant antimicrobial activities Okezie et al., 2016; Eleazu et al., 2013; Usman and Osuji, 2007; Prakash and Hosetti, 2010.The extract exhibited various antifungal activities in a dose dependent manner producing an inhibition of 8, 6, 4, & 2 mm against Tinea spp.1 at concentration of 400, 200, 100, and 50 mg/mL respectively. Also, the extract exhibited activity against Tinea spp.2 and Candida albicans having, a minimum inhibitory concentration of 100 and 200 mg/mL respectively. At a concentration of 400 mg/mL, the extract inhibited most of the test organism producing an inhibition zone of 8, 5, 4, 3, and 3 mm against Tinea spp.1, Tinea spp.2, Candida albicans, Aspergillus niger and Tinea spp.4 respectively. No antifungal activity was recorded against Tinea spp3, Tinea spp5 and Tinea spp6. Results of this study suggest that methanol extract of Dioscorea dumetorum (Pax) could be a promising source of novel antifungal compounds with pharmaceutical and industrial importance.
Comments
No have any comment !