Phytochemical Screening of Eugenia Caryophyllata (Clove Leaves) and Characterization of Its Essential Oil

  1. Home
  2. February 2018
Paul Madubuike

Phytochemical Screening of Eugenia Caryophyllata (Clove Leaves) and Characterization of Its Essential Oil

Keywords : Clove plant; Its Essential Oil; Analytical Characteristics.


Abstract

Qualitative Phytochemical screening were conducted on Clove plant using four different solvents and they include; Ethyl-acetate, Distilled Water, Methanol and N-Hexane as the extracting solvents and the of presence of Alkaloid, Tannin, Saponin, Flavonoid, Phenol, Steroids, Glycoside and Terpenoid were checked on the four different extracts and the results is as follows: Ethyl-acetate and Methanol extracts showed in abundance the presence of alkaloid(+ +), while N-Hexane and Water extracts showed the presence(+). N-Hexane extracts showed in abundance the presence of Tannin (+ +), while water and Ethyl-acetate extracts showed the presence of alkaloid, and Methanol extract showed none presence of Tannin (-). Methanol and water extracts showed the presence of Saponin in abundance (+ +), while N-hexane and Ethyl-acetate showed presence the Saponin (+). Methanol showed in abundance the presence the Flavanoids (+ +), while water and Ethyl-acetate extracts showed the presence of Flavonoids and N-Haxane showed none presence (-). However, Water extract show in abundance the presence of Phenol (+ +), followed by Ethyl-acetate and Methanol extracts which showed as well the presence of Phenol (+), while N-Haxane extracts showed none presence of Phenol (-). Meanwhile, the presence of Steroids were not found in any of the extracts.Glycoside’s presence were in the abundance in the extracts of Ethyl-acetate (+ +), Water and Methanol extracts (+ +), while N-Hexane showed presence of Glycoside (+). Terpenoid presence were in abundance (+ +) in Methanol extract, followed by Ethyl-acetate and water extracts which showed the presence of terpenoids(+), while N-Hexane extract showed none presence of Terpenoid. Physiochemical analysis results of Clove plant were as follows: Acid value 3.64(% as oleic acid), Saponification value 44.05 (MgKOH/g), Iodine value 9.0 (g I/100 of oil), Specific gravity 1.042, Refractive index 1.429, Free fatty acid 1.82 (% as oleic acid) Peroxide value 7.45 (Meq/Kg), Colour (Pt-Co) (Yellow) and Ester value 28.04( MgKOH/g). However, Mineral analysis results were as follows: Ca 0.600(mg/Kg), Mg 0.117(Mg/Kg), Mn 0.950(Mg/Kg), Fe 0.440(Mg/Kg), P 7.550(Mg/Kg), Na 0.001 (Mg/Kg), Zn 0.390(Mg/Kg), Cu 0.869(Mg/Kg), Pb 0.194(Mg/Kg) and K 0.039(Mg/Kg).TLC results as regards to distance traveled are as follows, Methanol, Ethyl-acetate, N-Hexane and Distilled water.

Download

Comments
No have any comment !
Leave a Comment