In order to confirm the presence or not of dengue virus in human blood samples, a study based on the epidemiological surveillance of dengue fever was conducted from December 2017 to March 2018 in hospitals located in areas which share borders with neighbouring countries in northern Benin.
Blood samples were collected monthly from patients with fever (>38°C) and presenting symptoms such as severe headache, retro-orbital pain, muscle, joint, nausea, vomiting, or rash.
Rapid diagnostic kits and the technique of Real Time PCR were used to diagnose dengue fever in these samples.
Results show that from the 1,569 samples coming from the various study sites and grouped into 169 pools of 140 μl each, only two samples from the Ouessè Health Center showed the presence of immunoglobulin type G suggesting the existence of a recent or old infection.
However, none of the 169 pools samples submitted to Real Time PCR were positive on dengue fever.
This work has highlighted the presence of immunoglobulin G (IgG) isotype of dengue fever in Benin.
It would be more important to carry out further studies between June and November of each year, a favorable period for the development of mosquitoes Aedes spp, responsible for dengue fever.
In the global context of reducing the level of malaria transmission, it would be desirable for physicians to be trained on the clinical symptoms of dengue fever, which symptoms are close to those of malaria. This will give an idea of the epidemiological level of dengue fever in Benin
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