Screening for Lysine Production by Bacteria Isolated From Nigerian Soil
- Country : Nigeria
- Subject : Microbiology
Keywords : Lysine production, Bacteria, Soil, Screening, Lysine auxotroph
Abstract
This study assessed seed coat dormancy of Enterolobium cyclocarpum using different pre-treatments. Seeds were sown in white plastic buckets filled with loose and well drained river sand at 2x 2cm spacing and at sowing depth of 3cm. Bucket diameter was 22 cm and bucket depth from base to the bream was 24 cm. 5 replications of 100 randomly picked seeds at 20 seeds per bucket was used for each treatment and were laid out in complete randomized design. Viable seeds determined by floatation method were subjected to pre-treatments using control, 98% concentrated Sulphuric acid and physical abrasion for 1, 2 and 3 minutes. Results shows that physical abrasion (55 to 58%) were most efficient with lowest mean emergence time of 15 to 18 days. Acid scarification (13 to 18%) with mean emergence time of 30 to 33 day and. wet heat (21 to 35%) with mean emergence time of 35 to 50 days. Control experiment gave 13% and mean emergence time of 53 days. Thus, 1, 2 and 3 minutes physical scarification is highly recommended for the propagation of Enterolobium cyclocarpum.
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