Isolation and characterization of virulent phages of Staphylococcus saprophyticus.
- Author Maia Elizbarashvili
- Co-Author Marina Darsavelidze, Tamar Suladze, Sophio Gunia, Marine Dadunashvili
- DOI 10.17605/O
- Country : Georgia
- Subject :
Keywords : Bacteriophages, bacterial strain, antibiotic resistance
Abstract
Staphylococcus saprophyticus is a Gram-positive, coagulase-negative microorganism belongs to Micrococcaceae family. It is a unique uropathogen associated with uncomplicated urinary tract infections (UTIs), the second most common pathogen after Escherichia coli. During our investigation 11 strains of S. saprophyticus isolated from patients with urinary tract infections were characterized with multiple antibiotic resistances - 73% of strains revealed resistance against β-lactam antibiotics, aminoglycosides and 91% of strains were resistance towards macrolides. From environmental source two new active phages - vB_S.s.1and vB_S.s.2 - against target pathogen S. saprophyticus have been isolated. Phages were belonged to Syphoviridae family, were characterized with high percentage of adsorption and big burst size. Formation of phage resistant mutant towards both phages was low.
Comments
No have any comment !