30 strains with stable characteristics (Staphyl.aureus -24, Staphyl.epidermidis-6) have been chosen from 102 laboratory and clinical cultures of staphylococci. Morphological, cultural and enzymatic activity, susceptibility to novobiocin and staphylococcal bacteriophage, ability to produce microcapsule, proteolytic, hemolytic characteristics, mannitol fermentation and plasmocoagulation values of strains have been studied. Producer animals were vaccinated with produced immunogens (thermoinactivated staphylococcal culture – 5 bn/ml; alpha-anatoxin, PV-leukocidin, hyaluronidase) according to the planned schedule. Immune serum has been obtained with highly specific activity. Antibody titer in Lh (Limes-hemolysis) reaction of alpha-toxin was - 150 IU/ml; antibacterial antibody titer in passive hemagglutination test - 1:6400-1:12800; anti-leukocidin antibodies - -1:320-1:1280; and antibody titer against hyaluronidase – 1:160-1:320; Normal and anti-staphylococcal immune serums were investigated with immunoenzyme method. Mean data from normal (control) strains was 0,081 for alpha-toxin, and antibody positivity index against α-toxin was 10,08 in immune serum, positivity index for antibacterial antibodies – 2, 3287.
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