The Separation of Proteins from media of Rat Osteoblast grown in the presence of biomaterials using Polyacrylamide Gel Electrophoresis
- Country : West Indies
- Subject : Biomaterials
Keywords : Biomaterials, Osteoblasts, Cell cultures, Biocompatible Wood, Implants, Medical Devices
Abstract
Osteoblasts harvested from calvaria from newly born Sprague-Dawley rats by enzyme digestion were cultured in a 24 well plate with the require media along with Co-Cr, PMMA, titanium implant fixtures, suture material and heat-treated wood. Growth media was sequentially removed at three, seven and ten days and subjected to one dimensional gel electrophoresis. At day three, there as a difference in protein expression between the materials. At day seven, there is greater uniformity in protein distribution seen for all materials. At day ten the media taken from wells containing cobalt-chrome, wood, and implant fixtures did not show a protein band corresponding to 24-25 kDa molecular weight. The experiments performed is sensitive enough to separate proteins consistent with a typical in vitro pre-clinical biocompatibility test. The results found here clearly indicate that the osteoblasts cells behave differently when grown with different materials.
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