Production of Extracellular Pectinase from Cultivable Soil Bacteria Strains

Leonardo Lima Bandeira

Production of Extracellular Pectinase from Cultivable Soil Bacteria Strains

  • Author Leonardo Lima Bandeira
  • Co-Author Larissa de Castro Viana, Ariel de Figueiredo Nogueira Mesquita, João Moreira de Matos Neto, Claudia Miranda Martins, Suzana Claudia Silveira Martins
  • DOI https://ww
Keywords : Pectin, Extracellular enzymes; Actinobacteria; Ecological services


Abstract

Among the abundant and highly interactive microbial groups present in soil, actinobacteria stand out. An extracellular enzyme type produced by this phylum of bacteria is pectinases, which are enzymes capable of degrading pectin complex biomolecules. The present study aimed to evaluate the pectinolytic activity of actinobacteria. Forty-five strains of actinobacteria were selected according to their morphological characteristics, area where they were isolated and diversity of genera. The pectinolytic enzymatic index (EI) for each strain was determined by the ratio between the hydrolysis halo diameter (Dh) and the colony diameter (Dc). Of the 45 actinobacteria strains evaluated in this study, 35 (77.8%) showed pectinase production, the five most productive having enzymatic indices greater than 6. One thing to be noticed in our study is that more than 30% of or strains can be classified as strong producers of pectinase, which show the ecological and biotechnological potential of strains analyzed.

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