Hepatitis B virus infection is one of the global public health problem, viral hepatitis especially B form a considerable percentage of liver disease worldwide. This study aims to determine the seroprevalence of hepatitis B virus among clients attending private medical laboratory diagnosis center in Karshi, Abuja, Nigeria. Blood samples were collected from clients and analysed to determine the presence of hepatitis B surface antigen (HBSAg) using Agary® HBSAg test kit. The study was carried out among 378 clients aged 11 and 50years between August, 2019 to March, 2020.Out of 378 clients screened 70(18.5%) were positive for hepatitis B surface antigen (HBSAg). Gender, age, education and marital status related prevalence of hepatitis B virus was determined. There was no statistically significant relationship between all the variables and prevalence of hepatitis B infection. The high prevalence of HBV infection amongst the clients studied strongly suggest that urgent preventive measures, particularly health education on HBV modes of transmission, prevention should be promoted and strengthened also compulsory universal vaccination of adults and children against HBV.
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