Study on the Use of Poultry Manure Extract in the Growth of Spirulina maxima with Reference to Indoor Culture.

Ankit Chaudhari

Study on the Use of Poultry Manure Extract in the Growth of Spirulina maxima with Reference to Indoor Culture.

Keywords : Spirulina maxima, Modified medium, Growth (Dry Weight), Specific growth rate, Chlorophyll.


Abstract

The effect of poultry manure supplementation on the development of Spirulina maxima was investigated for 18 days. The manure was collected from the poultry farm; after that, it was sun-dried and powdered by grinding it in a mixture grinder. 10-gram poultry manure powder was added to 100 ml tap water and it was steamed in a water bath for 30 minutes. The cultivation of Spirulina maxima was done by using the supplement of poultry manure extract in Spirulina growth medium (200ml) at different concentrations such as 0ml, 0.2ml, 1ml, 2ml, 3ml, and 4ml. Spirulina growth medium without any supplement of poultry manure extract was taken as a control. The experiment was done for 18 days and the culture was provided with a sufficient light source for 8 hours a day; the agitation was done by manual shaking 3-4 times a day throughout the experiment. The growth of Spirulina was measured as a wet weight and the dry weight of Spirulina by using the gravimetric method. The highest growth observed was 0.041gm in 200ml/2ml poultry manure extract. The highest specific growth rate observed was 0.0019 in 200ml/2ml poultry manure extract. Chlorophyll-a (10.6948 mg/L) and Chlorophyll-b (3.1641 mg/L) were found in higher concentrations at 200ml/1ml poultry manure extract. Chlorophyll-C (0.4142 mg/L) was high in control.

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