Bioremediation of Crude Oil Polluted Soil Using NPK 15:15:15 and Urea as a Biostimulant

Boluwatife S. Olubusoye

Bioremediation of Crude Oil Polluted Soil Using NPK 15:15:15 and Urea as a Biostimulant

Keywords : Biostimulant, Fertilizer, Bioremediation, Crude Oil, NPK, Urea


Abstract

Crude oil pollution is harmful and dangerous to plants, animals, and humans hence the need to decontaminate sites polluted by crude oil. In this study, the bioremediation of crude oil using organic fertilizer (Urea), and inorganic fertilizer (NPK) was investigated. This was done using 4kg of soil per sample polluted with 1L of crude per kg of soil. Soil samples were contaminated with crude oil in four different cells (cells undergoing natural attenuation as the control, Urea alone, NPK alone, and NPK + Urea). These combinations were added as biostimulants to the indigenous microorganism present in the contaminated soil. After eight weeks, the pH of the samples was approximately found to be within the range of 6-8 in all the cells, satisfying that stipulated by FEPA. Also, the cell undergoing natural attenuation has the highest residual hydrocarbon content (RHC) and total microbial count (TMC) values of 3577.81 mg/kg (% degradation of 44.29) and 9 x 104 Cfu/g respectively after eight weeks. While the mixture of NPK and Urea has the lowest RHC and TMC values of 790.83 mg/kg (% degradation of 87.48) and 9 x 106 Cfu/g respectively. From these results, this study showed that though remediation occurred in all the cells, the cells stimulated by nutrients and their combination offered better remediation results.

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