Isolation and Identification of Bioactive Compound from the Methanolic Leaf Extract of Eucalyptus torelliana
- Author Usman Mustapha Datin
- Co-Author I.Y Chindo, U.F Hassan
- DOI https://ww
- Country : Nigeria
- Subject : chemistry
Keywords : Eucalyptus torelliana, Methanolic Leaf Extract, Eucalyptus torelliana, Isolation and Identification
Abstract
The leaves of Eucalyptus torelliana are used in folklore medicine for the treatment of typhoid and pneumonia. In this study, Eucalyptus torelliana was investigated for the bioactive compounds present in its leaves. The plant was selected on the basis of its wide spread use in traditional herbal medicine. The leaves were collected from the plant with Bauchi metropolis in 2020. Extraction of the plant material was done with n-hexane, dichloromethane and methanol. The extracts obtained were subjected to phytochemical investigation using standard methods and revealed the presence of alkaloids, flavonoids, anthraquinones, tannins, saponins terpenoids and glycosides. The bioactivity of each extract was tested against Klebsiella pneumoniae and streptococcus pneumoniae. The bioactivity results indicated the presence of more bioactive compounds in the methanol extract compared to the dichloromethane and n-hexane extracts the probable reason might be the bioactive compounds in Eucalyptus torelliana leaves were more soluble in methanol extract (26 mm and 25 mm) compared to dichloromethane (15 mm and 5 mm) and n-hexane (no inhibition of growth). Separation and purification of compounds in the most active (methanol) extract was done using a combination of column chromatography and thin layer chromatography and this led to the isolation of one compound coded MD2 (Lupeol) with Rf value of 0.52. The compound was identified by combination of GC-MS and FTIR. The compound identified has a wide range of biological activity reported in literature review. This will justify the use of this plant in traditional medicine and indicates a promising potential for the development of medicinal agents from Eucalyptus torelliana leaves.
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