Characterization of virulent phages of Klebsiella pneumoniae.
- Author Marina Darsavelidze
- Co-Author Maia Elizbarashvili, Tamar Suladze, Sophio Gunia, Marine Dadunashvili
- DOI https://ww
- Country : Georgia
- Subject : Microbiology and Virology Gotua str
Keywords : K. pneumonia bacteriophage; phage genome; virion morphology; phage therapy.
Abstract
During this study six Klebsiella pneumoniae phages: vB_Kp6, vB_Kp8, vB_Kp4, vB_Kp4/1, vB_Kp19, vB_Kp19/1 were selected and studded. Based on TEM investigation was determined that phages belong to the Caudovirales order. Phages vB_KP6 and vB_KP8 belonged to the Siphoviridae family and phages vB_KP4, vB_KP4/1, vB_KP19 and vB_KP19/1 – to Myoviridae family. The range of activity of phages against K. pneumoniae - 92 and K. oxytoca - 9 strains were revealed that phages vB_Kp6 and vB_Kp8 were distinguished by the highest activity rate. Their range of activity was 60% and 59%, respectively. Based on the obtained results the biological properties (single reproduction cycle, frequency of formation of phage-resistant mutants, stability of lysis in the liquid area) and the influence of environmental factors (temperature and pH) of these two phages were investigated. As a result of the conducted work, these phages vB_Kp6 and vB_Kp8 can be recommended for creation a therapeutic-prophylactic phage preparation against Klebsiella infection.
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